DCAF11 Knockout cell line (Jurkat, Clone E6-1)

DCAF11 Knockout cell line (Jurkat, Clone E6-1) Order

Catalog#

YKO-H1152

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

DCAF11 Knockout cell line（Jurkat, Clone E6-1）

Cell Morphology

Lymphocyte-like,suspension

Passage ratio

1:3-1:5

Species

Human

Gene Name

DCAF11

Gene ID

80344

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%RPMI-1640+10%FBS

Target region

Knockout region: Exon 3 (based on transcript DCAF11-204)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:Jurkat, Clone E6-1			STR Info (Cell bank) Cell Line:Jurkat, Clone E6-1
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	9	9		9	9
D13S317	8	12		8	12
D7S820	8	12		8	12
D16S539	11	11		11	11
VWA	18	18		18	18
TH01	6	9.3		6	9.3
AMEL	X	Y		X	Y
TPOX	8	10		8	10
CSF1PO	11	12		11	12
D12S391	22	23
FGA	20	21
D2S1338	19	23
D21S11	31.2	33.2
D18S51	13	21	22.0
D8S1179	13	14
D3S1358	15	15
D6S1043	11	11
PENTAE	10	12
D19S433	14	15.2
PENTAD	11	13
D1S1656	16	17

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the DCAF11 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Jurkat, Clone E6-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, DCAF11 gene knockout DCAF11 Knockout cell line（Jurkat, Clone E6-1） will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

DCAF11 Knockout cell line (Jurkat, Clone E6-1)

Catalog#

YKO-H1152

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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DCAF11 Knockout cell line (Jurkat, Clone E6-1)

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